3O2G
Crystal Structure of Human gamma-butyrobetaine,2-oxoglutarate dioxygenase 1 (BBOX1)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-09-07 |
| Detector | ADSC QUANTUM 315 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 107.386, 107.386, 204.919 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.980 - 1.780 |
| R-factor | 0.14869 |
| Rwork | 0.147 |
| R-free | 0.17633 |
| Structure solution method | MIR |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.554 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | SHELXD |
| Refinement software | REFMAC (5.6.0066) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 84.690 |
| High resolution limit [Å] | 1.780 |
| Rmerge | 0.140 |
| Number of reflections | 43809 |
| <I/σ(I)> | 12.3 |
| Completeness [%] | 100.0 |
| Redundancy | 11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.2M AMMONIUM CITRATE, 20% PEG 3350, 6% DIAMINE HEXAN, 10MM ZNSO4, pH 7.0, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
