3O2E

Crystal structure of a bol-like protein from babesia bovis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2009-05-20
Detector	RIGAKU SATURN 944
Wavelength(s)	1.5418
Spacegroup name	P 41 21 2
Unit cell lengths	66.300, 66.300, 35.290
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.530 - 1.950
R-factor	0.185
Rwork	0.183
R-free	0.24100
Structure solution method	SAD
Starting model (for MR)	iodide SAD
RMSD bond length	0.018
RMSD bond angle	1.712
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	REFMAC (5.5.0109)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.000
High resolution limit [Å]	1.950	1.950
Rmerge	0.094	0.481
Number of reflections	6133
<I/σ(I)>	19.39	4.4
Completeness [%]	99.7	99.6
Redundancy	18.3	7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	290	EBS JCSG SCREEN E2: 2M AMMONIUM SULPHATE, 100MM CACODYLATE PH 6.5, 200MM NACL: BABOA.10365.A AT 12.4MG/ML, ENCUA.00735 AT 29.3MG/ML, PH N/A, VAPOR DIFFUSION, SITTING DROP, TEMPERATURE 290K