3O2C
Crystal structure of a rod form of c-phycocyanin from Themosynechococcus vulcanus at 1.5 angstroms
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-1 |
| Synchrotron site | ESRF |
| Beamline | ID14-1 |
| Wavelength(s) | 0.97950 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 187.469, 187.469, 60.180 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 26.000 - 1.500 |
| R-factor | 0.2403 |
| Rwork | 0.239 |
| R-free | 0.26310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.659 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA (3.3.9) |
| Phasing software | PHASER (2.1.4) |
| Refinement software | CNS (1.1) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 93.735 | 25.997 | 1.580 |
| High resolution limit [Å] | 1.500 | 4.740 | 1.500 |
| Rmerge | 0.007 | 0.204 | |
| Total number of observations | 8228 | 34212 | |
| Number of reflections | 63473 | ||
| <I/σ(I)> | 71.9 | 47.5 | 2.9 |
| Completeness [%] | 98.8 | 98.8 | 95.7 |
| Redundancy | 3.8 | 3.9 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 1.2M phosphate, pH 7, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
