3O2B
E. coli ClpS in complex with a Phe N-end rule peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.97 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 32.223, 58.405, 56.413 |
| Unit cell angles | 90.00, 101.89, 90.00 |
Refinement procedure
| Resolution | 30.200 - 2.050 |
| R-factor | 0.2126 |
| Rwork | 0.210 |
| R-free | 0.24660 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.007 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.7.2_869) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 30.200 |
| High resolution limit [Å] | 2.050 |
| Number of reflections | 13973 |
| Completeness [%] | 100.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 300 | 2 M Lithium Sulfate, 0.1 M TRIS, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 300K |
