3O1Z
High resolution crystal structure of histidine triad nucleotide-binding protein 1 (Hint1) double cysteine mutant from rabbit
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.1 |
Synchrotron site | BESSY |
Beamline | 14.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-09-17 |
Detector | RAYONIX MX-225 |
Wavelength(s) | 0.91841 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 39.790, 39.790, 142.630 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 26.170 - 1.300 |
R-factor | 0.13002 |
Rwork | 0.128 |
R-free | 0.17612 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3LLJ |
RMSD bond length | 0.024 |
RMSD bond angle | 2.039 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP (10.2.31) |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 26.170 | 1.370 |
High resolution limit [Å] | 1.300 | 1.300 |
Rmerge | 0.103 | 0.680 |
Number of reflections | 29103 | |
<I/σ(I)> | 14.2 | 4.1 |
Completeness [%] | 99.4 | 99 |
Redundancy | 12.4 | 12.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 281 | 30% PEG 8000, 0.1M sodium cacodylate pH 6.5, protein concentration 10 mg/ml, VAPOR DIFFUSION, HANGING DROP, temperature 281K |