3O1L
Crystal structure of a formyltetrahydrofolate deformylase (PSPTO_4314) from Pseudomonas syringae pv. tomato str. DC3000 at 2.20 A resolution
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL11-1 |
Synchrotron site | SSRL |
Beamline | BL11-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-06-11 |
Detector | MARMOSAIC 325 mm CCD |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 131.271, 131.271, 102.991 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.728 - 2.200 |
R-factor | 0.1745 |
Rwork | 0.173 |
R-free | 0.20230 |
Structure solution method | SAD |
RMSD bond length | 0.014 |
RMSD bond angle | 1.433 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SOLVE |
Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 29.728 | 29.728 | 2.280 |
High resolution limit [Å] | 2.200 | 4.720 | 2.200 |
Rmerge | 0.085 | 0.029 | 0.826 |
Number of reflections | 46149 | 8817 | 8829 |
<I/σ(I)> | 12.71 | 37.8 | 1.9 |
Completeness [%] | 99.8 | 99.4 | 99.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.57 | 293 | 1.4500M ammonium sulfate, 12.0000% Glycerol, 0.1M TRIS pH 8.57, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 293K |