3O1K
Crystal structure of putative dihydroneopterin aldolase (FolB) from Vibrio cholerae O1 biovar El Tor str. N16961
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-07-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9794 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 95.098, 101.734, 116.397 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 40.000 - 1.950 |
| R-factor | 0.19689 |
| Rwork | 0.194 |
| R-free | 0.24619 |
| Structure solution method | 2o90 |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.857 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 1.980 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.066 | 0.750 |
| Number of reflections | 41277 | |
| <I/σ(I)> | 30 | 2 |
| Completeness [%] | 99.9 | 99.4 |
| Redundancy | 6.8 | 5.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 5% isopropanon, 0.1 M Hepes, 10%Peg 4K, 25 % sucrose as cryo protectant, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 291K |
