3O1J
Crystal Structure of the TorS sensor domain - TorT complex in the absence of isopropanol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4C |
| Synchrotron site | NSLS |
| Beamline | X4C |
| Temperature [K] | 110 |
| Detector technology | IMAGE PLATE |
| Collection date | 2007-10 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 0.97900 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.390, 127.669, 306.534 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.960 - 2.950 |
| Rwork | 0.226 |
| R-free | 0.24900 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.400 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.950 |
| High resolution limit [Å] | 2.950 | 2.060 |
| Rmerge | 0.076 | 0.472 |
| Number of reflections | 63235 | |
| <I/σ(I)> | 6.6 | |
| Completeness [%] | 98.2 | 98.7 |
| Redundancy | 4.1 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277.15 | 4-7% isopropanol, 0.9-1.4M NH4Citrate pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 277.15K |
