3O1A
Structure of OxyE (CYP165D3), a Cytochrome P450 Involved in Teicoplanin Biosynthesis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2010-03-29 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.97824 |
| Spacegroup name | P 31 |
| Unit cell lengths | 75.680, 75.680, 74.720 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 49.270 - 2.500 |
| R-factor | 0.22286 |
| Rwork | 0.220 |
| R-free | 0.27366 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1lfk |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.184 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.600 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.080 | 0.570 |
| Number of reflections | 16371 | |
| <I/σ(I)> | 14.8 | 2.7 |
| Completeness [%] | 92.8 | 79.7 |
| Redundancy | 3.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1 M Bicine, 0.2 M NaCl, 16% (v/v) polyethylene glycol 20000, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
