3O19
Structure-function analysis of human L-Prostaglandin D Synthase bound with fatty acid
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 200 |
Detector technology | CCD |
Collection date | 2009-10-10 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.9794 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 60.512, 60.512, 177.230 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.602 - 1.660 |
R-factor | 0.1783 |
Rwork | 0.175 |
R-free | 0.24380 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2czu |
RMSD bond length | 0.010 |
RMSD bond angle | 1.423 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 1.720 |
High resolution limit [Å] | 1.660 | 1.660 |
Number of reflections | 23089 | |
Completeness [%] | 97.7 | 84.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 3.8 | 298 | 0.1M citric acid, pH 3.8, 1.7M ammonium sulfate, VAPOR DIFFUSION, HANGING DROP, temperature 298K |