3O18

Crystal structure of c-phycocyanin from Themosynechococcus vulcanus at 1.35 angstroms resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID23-1
Synchrotron site	ESRF
Beamline	ID23-1
Wavelength(s)	0.97950
Spacegroup name	H 3 2
Unit cell lengths	186.807, 186.807, 59.822
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	27.230 - 1.350
R-factor	0.2106
Rwork	0.210
R-free	0.22630
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1ktp
RMSD bond length	0.014
RMSD bond angle	1.280
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	CNS (1.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	93.404	2.130	1.420
High resolution limit [Å]	1.350	1.910	1.350
Rmerge	0.086	0.087	0.436
Number of reflections	86032
<I/σ(I)>	13.7	7.2	1.7
Completeness [%]	99.0	100	95.6
Redundancy	8.6	9.2	5.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	0.8M Ammonium sulfate, 10.5% sucrose, 50mM Tris , VAPOR DIFFUSION, HANGING DROP, temperature 293K