3O11
Anti-beta-amyloid antibody c706 fab in space group c2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2008-02-26 |
| Detector | RIGAKU SATURN 944 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 136.040, 45.020, 158.200 |
| Unit cell angles | 90.00, 92.69, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.800 |
| R-factor | 0.223 |
| Rwork | 0.220 |
| R-free | 0.30400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3mcl |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.347 |
| Data reduction software | d*TREK (9.6L) |
| Data scaling software | d*TREK (9.6L) |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 53.000 | 2.900 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Rmerge | 0.078 | 0.482 |
| Number of reflections | 20634 | |
| <I/σ(I)> | 12.3 | 2.4 |
| Completeness [%] | 85.4 | 82.3 |
| Redundancy | 6 | 6.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 3.5 | 293 | 0.1 M CITRATE PH 3.5, 18% PEG 8000 CRYO CONDITIONS, 0.1 M CITRATE PH 3.5, 25% PEG 8000, 20% GLYCEROL, VAPOR DIFFUSION, SITTING DROP, temperature 293K |
