3O0Z
Crystal structure of a coiled-coil domain from human ROCK I
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-09 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97914 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 84.588, 84.588, 340.129 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.133 - 2.330 |
R-factor | 0.2418 |
Rwork | 0.239 |
R-free | 0.28610 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.088 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.6_289)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.410 |
High resolution limit [Å] | 2.330 | 2.330 |
Rmerge | 0.120 | 0.334 |
Number of reflections | 69169 | |
<I/σ(I)> | 30.7 | 13.2 |
Completeness [%] | 67.6 | 14.5 |
Redundancy | 30.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.9 | 293 | 13% MPD, 100 mM NaCl, 100 mM sodium acetate, 10 mM DTT, 5% glycerol, 10 mM spermidine, pH 4.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |