3O0Y
The crystal structure of the putative lipoprotein from Colwellia psychrerythraea
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-04-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9794, 0.9796 |
| Spacegroup name | P 1 |
| Unit cell lengths | 67.954, 94.471, 93.894 |
| Unit cell angles | 114.32, 91.11, 100.43 |
Refinement procedure
| Resolution | 85.130 - 1.700 |
| R-factor | 0.14796 |
| Rwork | 0.146 |
| R-free | 0.18545 |
| Structure solution method | MAD |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.833 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 85.130 | 1.747 |
| High resolution limit [Å] | 1.700 | 1.703 |
| Rmerge | 0.086 | 0.623 |
| Number of reflections | 206348 | |
| <I/σ(I)> | 9.85 | 1 |
| Completeness [%] | 95.3 | 72.08 |
| Redundancy | 1.4 | 1.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 289 | 0.2M Lithium sulfate monohydrate, 0.1M Bis-tris pH5.5, 25% PEG3350, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
