3O08
Crystal structure of dimeric KlHxk1 in crystal form I
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | BESSY BEAMLINE 14.3 |
Synchrotron site | BESSY |
Beamline | 14.3 |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 2005-02-10 |
Detector | MAR scanner 345 mm plate |
Wavelength(s) | 0.8940 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 98.692, 113.219, 91.234 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.970 - 2.000 |
R-factor | 0.18587 |
Rwork | 0.185 |
R-free | 0.24366 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ig8 |
RMSD bond length | 0.024 |
RMSD bond angle | 2.017 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | AMoRE |
Refinement software | REFMAC (5.5.0109) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.050 |
High resolution limit [Å] | 2.000 | 2.000 |
Rmerge | 0.505 | |
Number of reflections | 69639 | |
<I/σ(I)> | 22 | 4.9 |
Completeness [%] | 99.8 | 98.9 |
Redundancy | 6.2 | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 9.4 | 292 | 1MICROL RESERVOIR + 1MICROL PROTEIN; RESERVOIR: 2.5M AMMONIA SULFATE, 0.1M CHES PH 9.4, PROTEIN: 10MG/ML KLHXK1, 10MM TRIS PH 7.4, 1MM EDTA, 1MM DTT, 0.5MM PMSF, VAPOR DIFFUSION, HANGING DROP, temperature 292K |