3O04

Crystal structure of the beta-keto-acyl carrier protein synthase II (lmo2201) from Listeria monocytogenes

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-F
Synchrotron site	APS
Beamline	21-ID-F
Temperature [K]	110
Detector technology	CCD
Collection date	2009-07-31
Detector	MARMOSAIC 225 mm CCD
Wavelength(s)	0.97872
Spacegroup name	P 43 21 2
Unit cell lengths	74.742, 74.742, 170.694
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.430 - 1.850
R-factor	0.1521
Rwork	0.151
R-free	0.17530
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.075
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHELXS
Refinement software	PHENIX (1.6.2_432)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.920
High resolution limit [Å]	1.850	3.990	1.850
Rmerge	0.081	0.041	0.629
Number of reflections	78477
<I/σ(I)>	21.9	40	2.9
Completeness [%]	99.6	98.6	99.3
Redundancy	6.4	6.3	6.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	298	2.5M Ammonium Sulfate, 100mM Bis-Tris Propane, pH 7, VAPOR DIFFUSION, HANGING DROP, temperature 298K