3O02
The Crystal Structure of the Salmonella Type III Secretion System Tip Protein SipD in Complex with Chenodeoxycholate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-BM |
| Synchrotron site | APS |
| Beamline | 17-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-08-12 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 202.216, 52.370, 57.316 |
| Unit cell angles | 90.00, 90.20, 90.00 |
Refinement procedure
| Resolution | 37.900 - 1.900 |
| R-factor | 0.19891 |
| Rwork | 0.197 |
| R-free | 0.24361 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.442 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.960 | 1.949 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.053 | |
| Number of reflections | 44253 | |
| <I/σ(I)> | 27.9 | |
| Completeness [%] | 97.9 | 81.97 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 25% (w/v) PEG 3350, 100mM Tris, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
