3O01
The Crystal Structure of the Salmonella Type III Secretion System Tip Protein SipD in Complex with Deoxycholate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 17-BM |
| Synchrotron site | APS |
| Beamline | 17-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-03-10 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 201.931, 52.327, 57.311 |
| Unit cell angles | 90.00, 90.34, 90.00 |
Refinement procedure
| Resolution | 25.330 - 1.900 |
| R-factor | 0.1992 |
| Rwork | 0.196 |
| R-free | 0.25678 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2p7n |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.387 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.980 | 1.950 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.053 | |
| Number of reflections | 44447 | |
| <I/σ(I)> | 20.3 | |
| Completeness [%] | 98.5 | 86.6 |
| Redundancy | 3.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.2 M magnesium formate dihydrate, 15% PEG 3350, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
