3NZN
The crystal structure of the Glutaredoxin from Methanosarcina mazei Go1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-03-07 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.9794 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 109.423, 31.542, 57.935 |
Unit cell angles | 90.00, 109.12, 90.00 |
Refinement procedure
Resolution | 51.690 - 1.100 |
R-factor | 0.14907 |
Rwork | 0.147 |
R-free | 0.18928 |
Structure solution method | SAD |
RMSD bond length | 0.031 |
RMSD bond angle | 2.637 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | HKL-3000 |
Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 51.690 | 1.129 |
High resolution limit [Å] | 1.100 | 1.100 |
Rmerge | 0.072 | 0.489 |
Number of reflections | 63391 | |
<I/σ(I)> | 21.24 | 1 |
Completeness [%] | 87.7 | 35.67 |
Redundancy | 4.2 | 1.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.2M (NH4)2SO4, 20% PEG3350, 0.1M Na-hepes, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |