3NZL

Crystal Structure of the N-terminal domain of DNA-binding protein SATB1 from Homo sapiens, Northeast Structural Genomics Consortium Target HR4435B

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X4A
Synchrotron site	NSLS
Beamline	X4A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-30
Detector	ADSC QUANTUM 4
Spacegroup name	P 21 21 21
Unit cell lengths	33.926, 43.480, 42.461
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.631 - 1.199
R-factor	0.1505
Rwork	0.148
R-free	0.17700
Structure solution method	SAD
RMSD bond length	0.010
RMSD bond angle	1.219
Data reduction software	HKL-2000
Data scaling software	SCALEPACK
Phasing software	SHELX (FOLLOWED BY SOLVE/RESOLVE)
Refinement software	PHENIX (1.6.4_486)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.350
High resolution limit [Å]	1.199	1.199
Rmerge	0.037	0.076
Number of reflections	23439
<I/σ(I)>	35.5	14.7
Completeness [%]	78.3	59.7
Redundancy	4.5	3.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	Protein solution: 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl (pH 7.5), Reservoir solution: 0.1M Tris (pH 7.5) and 20% PEG 3350, VAPOR DIFFUSION, HANGING DROP, temperature 277K