3NZE
The crystal structure of a domain of a possible sugar-binding transcriptional regulator from Arthrobacter aurescens TC1.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97929 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.337, 86.286, 64.641 |
| Unit cell angles | 90.00, 94.75, 90.00 |
Refinement procedure
| Resolution | 36.000 - 1.697 |
| R-factor | 0.1698 |
| Rwork | 0.168 |
| R-free | 0.19910 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.026 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | SHELXD |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.094 | 0.361 |
| Number of reflections | 49143 | |
| <I/σ(I)> | 14.8 | 2 |
| Completeness [%] | 98.1 | 84.6 |
| Redundancy | 3.2 | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 289 | 10% 2-propanol, 0.1M MES, 0.2M calcium acetate, pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
