3NYK
The structure of cobalt-substituted pseudoazurin from Alcaligenes faecalis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH3R |
| Temperature [K] | 291 |
| Detector technology | IMAGE PLATE |
| Collection date | 2010-05-11 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 65 |
| Unit cell lengths | 50.033, 50.033, 98.803 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.330 - 1.860 |
| R-factor | 0.1509 |
| Rwork | 0.150 |
| R-free | 0.17806 |
| Structure solution method | SAD |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.449 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | Patterson (search) |
| Refinement software | REFMAC (5.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.330 | 1.930 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Rmerge | 0.038 | 0.073 |
| Number of reflections | 11749 | |
| <I/σ(I)> | 14.5 | 8.8 |
| Completeness [%] | 99.2 | 98.6 |
| Redundancy | 10.3 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.7 | Protein at 5 mg/ml concentration. 50mM Na-citrate pH 5.7, 20 mM CoCl2, 2.8 M ammonium sulfate, VAPOR DIFFUSION, SITTING DROP |
