3NYB

Structure and function of the polymerase core of TRAMP, a RNA surveillance complex

Experimental procedure

Experimental method	SAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	110
Detector technology	CCD
Collection date	2009-04-19
Detector	ADSC QUANTUM 315
Wavelength(s)	1.28215
Spacegroup name	P 3 2 1
Unit cell lengths	92.720, 92.720, 103.630
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.656 - 2.701
R-factor	0.1982
Rwork	0.194
R-free	0.25810
Structure solution method	SAD
RMSD bond length	0.006
RMSD bond angle	1.034
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	SHARP
Refinement software	PHENIX ((phenix.refine: 1.5_2))

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	25.000	2.800	2.690
High resolution limit [Å]	2.600	2.690	2.600
Rmerge	0.077	0.109	0.082
Number of reflections	16163
<I/σ(I)>		4.3	2.3
Completeness [%]	100.0	100	100
Redundancy	10.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.8	277	100 mM sodium citrate at pH 5.8, 200 mM sodium acetate, 11% PEG 4000, VAPOR DIFFUSION, HANGING DROP, temperature 277K