3NY1
Structure of the ubr-box of the UBR1 ubiquitin ligase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F2 |
| Synchrotron site | CHESS |
| Beamline | F2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-10-28 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.2836 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 29.702, 49.262, 43.831 |
| Unit cell angles | 90.00, 100.51, 90.00 |
Refinement procedure
| Resolution | 43.100 - 2.085 |
| R-factor | 0.19673 |
| Rwork | 0.194 |
| R-free | 0.24783 |
| Structure solution method | SAD |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.471 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | SOLVE |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.120 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Number of reflections | 7043 | |
| Completeness [%] | 98.7 | 86.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1M Bis-Tris, 25% PEG3350, pH 6.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
