3NXS
Crystal structure of LAO/AO transport system from Mycobacterium smegmatis bound to GDP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-06-25 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 78.630, 91.460, 57.470 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 50.000 - 2.300 |
R-factor | 0.2098 |
Rwork | 0.209 |
R-free | 0.23120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3md0 |
RMSD bond length | 0.015 |
RMSD bond angle | 1.501 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER (2.1.4) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.360 | |
High resolution limit [Å] | 2.300 | 10.290 | 2.300 |
Rmerge | 0.054 | 0.021 | 0.489 |
Number of reflections | 18658 | 217 | 1325 |
<I/σ(I)> | 22.87 | 49.3 | 4.3 |
Completeness [%] | 98.2 | 83.5 | 97.9 |
Redundancy | 8.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 9 | 289 | JCSG+ condition F6, 0.1% Bicine pH 9.0, 10% MPD as crystallant, 28.66 mg/mL protein, crystal tracking ID 215415f6, VAPOR DIFFUSION, SITTING DROP, temperature 289K |