3NXL
Crystal structure of Glucarate dehydratase from Burkholderia cepacia complexed with magnesium
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X4A |
| Synchrotron site | NSLS |
| Beamline | X4A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-04-29 |
| Detector | ADSC QUANTUM 4 |
| Wavelength(s) | 0.97915 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 165.289, 121.452, 108.652 |
| Unit cell angles | 90.00, 92.60, 90.00 |
Refinement procedure
| Resolution | 34.235 - 1.885 |
| R-factor | 0.1917 |
| Rwork | 0.190 |
| R-free | 0.22070 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.984 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.5_2)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 34.235 |
| High resolution limit [Å] | 1.885 |
| Number of reflections | 167926 |
| Completeness [%] | 97.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | 20% PEG 3350,0.2M sodium malonate, pH 7.0, VAPOR DIFFUSION, HANGING DROP, temperature 293.0K |
