3NXK
Crystal Structure of Probable Cytoplasmic L-asparaginase from Campylobacter jejuni
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-BM |
| Synchrotron site | APS |
| Beamline | 19-BM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-03-07 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 0.97911 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 73.321, 127.653, 149.832 |
| Unit cell angles | 90.00, 103.05, 90.00 |
Refinement procedure
| Resolution | 36.911 - 2.400 |
| R-factor | 0.169 |
| Rwork | 0.166 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PDBID 1NNS |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.117 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine: 1.6.2_432)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Number of reflections | 104817 | |
| <I/σ(I)> | 6.6 | 2.32 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 4 | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 0.2 M Ammonium Sulfate, 20% PEG 3350, pH 7.5, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
