3NXG
JC polyomavirus VP1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2008-08-30 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9506 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 149.570, 96.020, 128.550 |
| Unit cell angles | 90.00, 110.44, 90.00 |
Refinement procedure
| Resolution | 19.830 - 1.950 |
| R-factor | 0.17545 |
| Rwork | 0.174 |
| R-free | 0.20658 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.102 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.5.0102) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.000 |
| High resolution limit [Å] | 1.950 | 1.950 |
| Rmerge | 0.093 | 0.324 |
| Number of reflections | 119853 | |
| <I/σ(I)> | 10.9 | 2.5 |
| Completeness [%] | 96.5 | 91.7 |
| Redundancy | 3.5 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 12 % PEG 3,350, 0.1 M HEPES, 0.2 M KSCN, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
