3NXC
Molecular mechanism by which the Escherichia coli nucleoid occlusion factor, SlmA, keeps cytokinesis in check
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.3.1 |
| Synchrotron site | ALS |
| Beamline | 8.3.1 |
| Temperature [K] | 298 |
| Detector technology | CCD |
| Collection date | 2008-10-12 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.9795, 0.97953, 0.95372 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 50.380, 50.380, 121.320 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 43.727 - 2.500 |
| R-factor | 0.224 |
| Rwork | 0.224 |
| R-free | 0.26500 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.470 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.727 | 2.500 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.109 | 0.421 |
| Number of reflections | 6647 | |
| <I/σ(I)> | 18.8 | 4.1 |
| Completeness [%] | 99.4 | 99.4 |
| Redundancy | 6.6 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 287 | 100 mM Tris pH 8.5, 10% PEG 400, 58 mM LiSO4, VAPOR DIFFUSION, HANGING DROP, temperature 287K |
