3NX6
Crystal Structure of co-chaperonin, GroES (Xoo4289) from Xanthomonas oryzae pv. oryzae KACC10331
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 4A |
Synchrotron site | PAL/PLS |
Beamline | 4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-06-11 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.00000 |
Spacegroup name | P 61 |
Unit cell lengths | 64.410, 64.410, 36.500 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 32.210 - 1.970 |
R-factor | 0.21722 |
Rwork | 0.214 |
R-free | 0.29163 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1wnr |
RMSD bond length | 0.027 |
RMSD bond angle | 2.170 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | MOLREP |
Refinement software | REFMAC (5.5.0072) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 55.780 | 2.080 |
High resolution limit [Å] | 1.970 | 1.970 |
Number of reflections | 5590 | |
Completeness [%] | 94.5 | 98.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 4.1 | 287 | 0.2M Sodium citrate pH 4.1, 16% PEG 400, 0.2M Li2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 287K |