3NX6

Crystal Structure of co-chaperonin, GroES (Xoo4289) from Xanthomonas oryzae pv. oryzae KACC10331

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 4A
Synchrotron site	PAL/PLS
Beamline	4A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-06-11
Detector	ADSC QUANTUM 210
Wavelength(s)	1.00000
Spacegroup name	P 61
Unit cell lengths	64.410, 64.410, 36.500
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.210 - 1.970
R-factor	0.21722
Rwork	0.214
R-free	0.29163
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1wnr
RMSD bond length	0.027
RMSD bond angle	2.170
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	MOLREP
Refinement software	REFMAC (5.5.0072)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.780	2.080
High resolution limit [Å]	1.970	1.970
Number of reflections	5590
Completeness [%]	94.5	98.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	4.1	287	0.2M Sodium citrate pH 4.1, 16% PEG 400, 0.2M Li2SO4, VAPOR DIFFUSION, HANGING DROP, temperature 287K