3NX3
Crystal structure of acetylornithine aminotransferase (argD) from Campylobacter jejuni
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 110 |
Detector technology | CCD |
Collection date | 2010-03-17 |
Detector | MARMOSAIC 225 mm CCD |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 51.110, 66.160, 214.340 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 29.400 - 1.800 |
R-factor | 0.1552 |
Rwork | 0.153 |
R-free | 0.19610 |
Structure solution method | SAD |
RMSD bond length | 0.007 |
RMSD bond angle | 1.040 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELXS |
Refinement software | PHENIX (1.6.2_432) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 29.400 | 1.810 |
High resolution limit [Å] | 1.750 | 1.750 |
Rmerge | 0.067 | 0.501 |
Number of reflections | 73891 | |
<I/σ(I)> | 21.34 | 4.62 |
Completeness [%] | 99.3 | 95.9 |
Redundancy | 8.74 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 295 | 20% PEG3350, 200mM magnesium chloride, 100mM Hepes pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 295K |