3NX2
Enterobacter sp. Px6-4 Ferulic Acid Decarboxylase in complex with substrate analogues
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2009-01-30 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.334, 88.740, 49.017 |
| Unit cell angles | 90.00, 102.30, 90.00 |
Refinement procedure
| Resolution | 25.170 - 2.010 |
| R-factor | 0.19129 |
| Rwork | 0.189 |
| R-free | 0.23588 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3nx1 |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.898 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.010 |
| Number of reflections | 22288 |
| Completeness [%] | 98.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.1M HEPES pH7.5, 20% (w/v) PEG 10000, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
