3NX1
Crystal structure of Enterobacter sp. Px6-4 Ferulic Acid Decarboxylase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2008-12-21 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.477, 88.715, 49.264 |
| Unit cell angles | 90.00, 102.25, 90.00 |
Refinement procedure
| Resolution | 36.870 - 2.400 |
| R-factor | 0.16668 |
| Rwork | 0.162 |
| R-free | 0.26163 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2gc9 |
| RMSD bond length | 0.036 |
| RMSD bond angle | 2.954 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.400 |
| Number of reflections | 12090 |
| Completeness [%] | 99.0 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 291 | 0.1M HEPES pH 7.5, 20%(w/v) PEG 10000, VAPOR DIFFUSION, HANGING DROP, temperature 291K |
