3NWZ
Crystal Structure of BH2602 protein from Bacillus halodurans with CoA, Northeast Structural Genomics Consortium Target BhR199
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X4A |
Synchrotron site | NSLS |
Beamline | X4A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2010-06-30 |
Detector | ADSC QUANTUM 4 |
Wavelength(s) | 0.97901 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 52.243, 89.733, 71.850 |
Unit cell angles | 90.00, 107.37, 90.00 |
Refinement procedure
Resolution | 27.883 - 2.566 |
R-factor | 0.2326 |
Rwork | 0.231 |
R-free | 0.26390 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.331 |
Refinement software | PHENIX (1.6.1_357) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 30.000 |
High resolution limit [Å] | 2.600 |
Rmerge | 0.080 |
Number of reflections | 38995 |
<I/σ(I)> | 18 |
Completeness [%] | 99.1 |
Redundancy | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | Protein solution: 100mM NaCl, 5mM DTT, 0.02% NaN3, 10mM Tris-HCl (pH 7.5), Reservoir solution: 0.2M ammonium sulfate, 0.1M hepes, 18% PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 293K |