3NWS
Crystal structure of the N-terminal domain of the yeast telomere-binding and telomerase regulatory protein Cdc13
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X6A |
Synchrotron site | NSLS |
Beamline | X6A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-06-21 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.978 |
Spacegroup name | P 1 |
Unit cell lengths | 61.964, 66.866, 68.138 |
Unit cell angles | 116.22, 90.29, 94.72 |
Refinement procedure
Resolution | 20.000 - 2.500 |
R-factor | 0.25244 |
Rwork | 0.245 |
R-free | 0.28788 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.014 |
RMSD bond angle | 1.295 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.540 |
High resolution limit [Å] | 2.500 | 2.500 |
Number of reflections | 29636 | |
<I/σ(I)> | 13.8 | 2 |
Completeness [%] | 93.1 | 94.2 |
Redundancy | 1.9 | 1.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 10% PEG 3,350K, 0.01 M magnesium chloride hexahydrate and 50 mM Hepes (pH 7.0), VAPOR DIFFUSION, SITTING DROP, temperature 291K |