3NWS
Crystal structure of the N-terminal domain of the yeast telomere-binding and telomerase regulatory protein Cdc13
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X6A |
| Synchrotron site | NSLS |
| Beamline | X6A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-06-21 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.978 |
| Spacegroup name | P 1 |
| Unit cell lengths | 61.964, 66.866, 68.138 |
| Unit cell angles | 116.22, 90.29, 94.72 |
Refinement procedure
| Resolution | 20.000 - 2.500 |
| R-factor | 0.25244 |
| Rwork | 0.245 |
| R-free | 0.28788 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.295 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.540 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Number of reflections | 29636 | |
| <I/σ(I)> | 13.8 | 2 |
| Completeness [%] | 93.1 | 94.2 |
| Redundancy | 1.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 10% PEG 3,350K, 0.01 M magnesium chloride hexahydrate and 50 mM Hepes (pH 7.0), VAPOR DIFFUSION, SITTING DROP, temperature 291K |
