3NWI
The Soluble Domain Structure of the ZntB Zn2+ Efflux System
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 14-ID-B |
Synchrotron site | APS |
Beamline | 14-ID-B |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.282 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 173.570, 101.944, 90.288 |
Unit cell angles | 90.00, 110.42, 90.00 |
Refinement procedure
Resolution | 48.567 - 3.130 |
R-factor | 0.2168 |
Rwork | 0.213 |
R-free | 0.26570 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.010 |
RMSD bond angle | 1.282 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.000 | 3.170 |
High resolution limit [Å] | 3.130 | 3.130 |
Number of reflections | 25929 | |
<I/σ(I)> | 2.2 | |
Completeness [%] | 99.0 | 100 |
Redundancy | 3.8 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 30-35% PEG 3350, 0.1 M Tris pH8.5, 0.2 M (NH4)2SO4, 150 mM NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 277K |