3NWI
The Soluble Domain Structure of the ZntB Zn2+ Efflux System
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.282 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 173.570, 101.944, 90.288 |
| Unit cell angles | 90.00, 110.42, 90.00 |
Refinement procedure
| Resolution | 48.567 - 3.130 |
| R-factor | 0.2168 |
| Rwork | 0.213 |
| R-free | 0.26570 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.282 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.000 | 3.170 |
| High resolution limit [Å] | 3.130 | 3.130 |
| Number of reflections | 25929 | |
| <I/σ(I)> | 2.2 | |
| Completeness [%] | 99.0 | 100 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 277 | 30-35% PEG 3350, 0.1 M Tris pH8.5, 0.2 M (NH4)2SO4, 150 mM NaCl, VAPOR DIFFUSION, HANGING DROP, temperature 277K |
