3NWF
Glycoprotein B from Herpes simplex virus type 1, low-pH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2003-09-13 |
Detector | ADSC QUANTUM 270 |
Wavelength(s) | 0.9186 |
Spacegroup name | P 3 |
Unit cell lengths | 117.802, 117.802, 318.324 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 41.535 - 3.000 |
R-factor | 0.1994 |
Rwork | 0.197 |
R-free | 0.24920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3nwa |
RMSD bond length | 0.003 |
RMSD bond angle | 0.658 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.367 | 2.900 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.125 | |
Number of reflections | 112550 | |
<I/σ(I)> | 8.98 | 0.98 |
Completeness [%] | 92.5 | 68 |
Redundancy | 3.5 | 2.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.5 | 293 | 15% PEG 4000, 0.3M NaCl, and 0.1M sodium citrate, pH 5.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |