3NWC

Crystal Structure of the Pyrococcus furiosus SMC Protein Hinge Domain

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06SA
Synchrotron site	SLS
Beamline	X06SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2007-09-03
Detector	PSI PILATUS 6M
Wavelength(s)	0.9788, 0.9794, 0.9778
Spacegroup name	C 2 2 21
Unit cell lengths	69.708, 118.653, 82.637
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	33.906 - 1.696
R-factor	0.1981
Rwork	0.194
R-free	0.23520
Structure solution method	MAD
RMSD bond length	0.006
RMSD bond angle	0.991
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	SHARP
Refinement software	PHENIX (1.6.1_357)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	35.000	35.000	1.800
High resolution limit [Å]	1.696	5.040	1.696
Rmerge	0.046	0.033	0.336
Rmeas		0.036	0.365
Number of reflections	38299	2793	11582
<I/σ(I)>	24.02	57	4.7
Completeness [%]	99.6	99.5	97.7
Redundancy	6.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	1.8M ammonium sulphate, 0.2M potassium-sodium tartrate, 0.1M trisodium citrate pH 6.0, vapor diffusion, hanging drop, temperature 293K