3NWC
Crystal Structure of the Pyrococcus furiosus SMC Protein Hinge Domain
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2007-09-03 |
| Detector | PSI PILATUS 6M |
| Wavelength(s) | 0.9788, 0.9794, 0.9778 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 69.708, 118.653, 82.637 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.906 - 1.696 |
| R-factor | 0.1981 |
| Rwork | 0.194 |
| R-free | 0.23520 |
| Structure solution method | MAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.991 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | SHARP |
| Refinement software | PHENIX (1.6.1_357) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 35.000 | 35.000 | 1.800 |
| High resolution limit [Å] | 1.696 | 5.040 | 1.696 |
| Rmerge | 0.046 | 0.033 | 0.336 |
| Rmeas | 0.036 | 0.365 | |
| Number of reflections | 38299 | 2793 | 11582 |
| <I/σ(I)> | 24.02 | 57 | 4.7 |
| Completeness [%] | 99.6 | 99.5 | 97.7 |
| Redundancy | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 1.8M ammonium sulphate, 0.2M potassium-sodium tartrate, 0.1M trisodium citrate pH 6.0, vapor diffusion, hanging drop, temperature 293K |
