3NW8
Glycoprotein B from Herpes simplex virus type 1, Y179S mutant, high-pH
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X25 |
Synchrotron site | NSLS |
Beamline | X25 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2008-08-26 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0809 |
Spacegroup name | P 3 |
Unit cell lengths | 117.985, 117.985, 321.537 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.690 - 2.759 |
R-factor | 0.2037 |
Rwork | 0.202 |
R-free | 0.24250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3nwa |
RMSD bond length | 0.003 |
RMSD bond angle | 0.601 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.6.1_357)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.690 | 2.810 |
High resolution limit [Å] | 2.759 | 2.760 |
Rmerge | 0.115 | |
Number of reflections | 118749 | |
<I/σ(I)> | 12.9 | 1.38 |
Completeness [%] | 92.1 | 83 |
Redundancy | 4.5 | 3.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 10% PEG 4000, 0.2M NaCl, 0.1M Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |