3NVO
The Soluble Domain Structure of the ZntB Zn2+ Efflux System
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MAR CCD 165 mm |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 45.039, 93.845, 68.231 |
Unit cell angles | 90.00, 91.58, 90.00 |
Refinement procedure
Resolution | 38.658 - 2.300 |
R-factor | 0.204 |
Rwork | 0.201 |
R-free | 0.25610 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.005 |
RMSD bond angle | 0.834 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.390 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 24331 | |
Completeness [%] | 96.0 | 74.4 |
Redundancy | 3.5 | 2.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 |