3NVI

Structure of N-terminal truncated Nop56/58 bound with L7Ae and box C/D RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	MAR scanner 300 mm plate
Spacegroup name	P 21 21 21
Unit cell lengths	87.216, 91.841, 155.603
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	31.621 - 2.709
R-factor	0.21
Rwork	0.208
R-free	0.24870
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.341
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX ((phenix.refine))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	50.000
High resolution limit [Å]	2.700	2.700
Number of reflections	29910
Completeness [%]	86.4	86.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	303	200 mM potassium chloride, 5 mM magnesium chloride, 50 mM TrisHCl (pH 7.5), and 4%- 8% PEG4000 or PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 303K
1	VAPOR DIFFUSION, HANGING DROP	6.5	303	200 mM potassium chloride, 5 mM magnesium chloride, 50 mM TrisHCl (pH 7.5), and 4%- 8% PEG4000 or PEG3350, VAPOR DIFFUSION, HANGING DROP, temperature 303K