3NVH
MIHFGND segment 137-143 from mouse prion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID13 |
| Synchrotron site | ESRF |
| Beamline | ID13 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-12-18 |
| Detector | MAR CCD 165 |
| Wavelength(s) | 0.9466 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 30.280, 4.873, 31.107 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.700 - 1.610 |
| R-factor | 0.169 |
| Rwork | 0.166 |
| R-free | 0.19720 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.297 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (1.3.2) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 90.000 | 90.000 | 1.710 |
| High resolution limit [Å] | 1.610 | 3.550 | 1.610 |
| Rmerge | 0.206 | 0.157 | 0.280 |
| Number of reflections | 714 | ||
| <I/σ(I)> | 7 | ||
| Completeness [%] | 97.3 | 100 | 88.7 |
| Redundancy | 6.3 | 8.1 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 100mM HEPES ph 7.0, 10% PEG 1200, vapor diffusion, hanging drop, temperature 298K |
