3NVG
MIHFGN segment 137-142 from mouse prion
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2006-11-20 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 1.0048 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 27.280, 4.771, 31.476 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.620 - 1.480 |
| R-factor | 0.1835 |
| Rwork | 0.181 |
| R-free | 0.20490 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.346 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | PHASER (1.3.2) |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 90.000 | 90.000 | 1.620 |
| High resolution limit [Å] | 1.480 | 2.560 | 1.480 |
| Rmerge | 0.154 | 0.108 | 0.452 |
| Number of reflections | 801 | ||
| <I/σ(I)> | 7 | ||
| Completeness [%] | 90.9 | 99.5 | 68.8 |
| Redundancy | 5.3 | 5.3 | 3.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 100mM HEPES 7.5, vapor diffusion, hanging drop, temperature 298K |
