3NUF
Crystal structure of a PRD-containing transcription regulator (LSEI_2718) from Lactobacillus casei ATCC 334 at 1.38 A resolution
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-11-05 |
Detector | MARMOSAIC 325 mm CCD |
Wavelength(s) | 0.91837,0.97954,0.97939 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 67.160, 67.160, 99.733 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 28.759 - 1.380 |
R-factor | 0.172 |
Rwork | 0.171 |
R-free | 0.19200 |
Structure solution method | MAD |
RMSD bond length | 0.018 |
RMSD bond angle | 1.567 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | SHELX |
Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 28.759 | 28.759 | 1.430 |
High resolution limit [Å] | 1.380 | 2.970 | 1.380 |
Rmerge | 0.054 | 0.028 | 0.877 |
Number of reflections | 47575 | 9021 | 8733 |
<I/σ(I)> | 13.65 | 41 | 1.7 |
Completeness [%] | 99.4 | 99.4 | 96 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | 1.6000M ammonium sulfate, 4.0000% polyethylene glycol 400, 0.1M HEPES pH 7.5, NANODROP, VAPOR DIFFUSION, SITTING DROP, temperature 277K |