3NTX
Crystal Structure of L-asparaginase I from Yersinia pestis
Experimental procedure
| Experimental method | SAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-02-28 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97929 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 125.377, 109.089, 58.224 |
| Unit cell angles | 90.00, 117.63, 90.00 |
Refinement procedure
| Resolution | 38.915 - 1.900 |
| R-factor | 0.156 |
| Rwork | 0.154 |
| R-free | 0.19200 |
| Structure solution method | SAD |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.449 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | HKL-3000 |
| Refinement software | PHENIX ((phenix.refine: 1.6.2_432)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.920 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Number of reflections | 54312 | |
| <I/σ(I)> | 11.3 | 2.5 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4.9 | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 289 | 0.16 M magnesium chloride, 0.08 M Tris HCl pH 8.5, 24 %w/v PEG4000, 20 % v/v glycerol, VAPOR DIFFUSION, SITTING DROP, temperature 289K |
