3NTW
Structure of the MLLE domain of EDD in complex with a PAM2 peptide from Paip1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE A1 |
| Synchrotron site | CHESS |
| Beamline | A1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2007-11-04 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 0.9779 |
| Spacegroup name | P 64 2 2 |
| Unit cell lengths | 95.794, 95.794, 82.936 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 41.490 - 2.600 |
| R-factor | 0.22988 |
| Rwork | 0.227 |
| R-free | 0.28994 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i2t |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.060 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.660 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.054 | 0.343 |
| Number of reflections | 6999 | |
| <I/σ(I)> | 24.2 | 6.6 |
| Completeness [%] | 99.8 | 99.81 |
| Redundancy | 10.1 | 8.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 1.0 M ammonium sulfate, 0.2 M lithium sulfate, 10% glycerol and 0.1 M Tris-HCl, pH 8.5, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
