3NTU
RADA RECOMBINASE D302K MUTANT IN COMPLEX with AMP-PNP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2010-05-20 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 61 |
| Unit cell lengths | 84.970, 84.970, 104.320 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 1.900 |
| R-factor | 0.188 |
| Rwork | 0.188 |
| R-free | 0.21400 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 1t4g |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.730 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.980 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.049 | 0.278 |
| Number of reflections | 33350 | |
| <I/σ(I)> | 27.1 | 10 |
| Completeness [%] | 99.1 | 98.6 |
| Redundancy | 9.5 | 9.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 2mM AMP-PNP, 4-5% PEG 3350, 50 MM MGCL2, 0.4 M NACL, 50 MM TRIS-HCL and 14% sucrose, pH 6-8, VAPOR DIFFUSION, HANGING DROP, temperature 294.0K |
