3NTT
Structural insights of Adeno-Associated virus 5. A gene therapy Vector for Cystic Fibrosis
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS BEAMLINE X29A |
Synchrotron site | NSLS |
Beamline | X29A |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 264.700, 447.900, 629.700 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.959 - 3.450 |
R-factor | 0.2515 |
Rwork | 0.252 |
R-free | 0.25150 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2g8g |
RMSD bond length | 0.005 |
RMSD bond angle | 1.015 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.570 |
High resolution limit [Å] | 3.450 | 3.450 |
Number of reflections | 762314 | |
<I/σ(I)> | 7.9 | |
Completeness [%] | 78.6 | 58.5 |
Redundancy | 2.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 20mM Tris HCL, 350mM NaCl, MgCl2, 1.5% PEK 8K, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 20mM Tris HCL, 350mM NaCl, MgCl2, 1.5% PEK 8K, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |