3NTK
Crystal structure of Tudor
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X29A |
| Synchrotron site | NSLS |
| Beamline | X29A |
| Temperature [K] | 100 |
| Wavelength(s) | 1.0809 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 104.145, 47.262, 85.404 |
| Unit cell angles | 90.00, 106.24, 90.00 |
Refinement procedure
| Resolution | 50.000 - 1.800 |
| Rwork | 0.210 |
| R-free | 0.23780 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.382 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.860 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.056 | 0.236 |
| Number of reflections | 35907 | |
| <I/σ(I)> | 2.4 | |
| Completeness [%] | 96.2 | 80.3 |
| Redundancy | 3.3 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 1.2M sodium citrate, VAPOR DIFFUSION, HANGING DROP, temperature 289K | |
| 1 | VAPOR DIFFUSION, HANGING DROP | 289 | 1.2M sodium citrate, VAPOR DIFFUSION, HANGING DROP, temperature 289K |
