3NTE
Crystal Structure of the Wild-type Full-Length HIV-1 Capsid Protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL9-2 |
Synchrotron site | SSRL |
Beamline | BL9-2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2009-01-24 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 1.0 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 47.910, 86.532, 55.589 |
Unit cell angles | 90.00, 99.73, 90.00 |
Refinement procedure
Resolution | 29.048 - 1.950 |
R-factor | 0.2064 |
Rwork | 0.204 |
R-free | 0.25340 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB entries 1ak4 1a43 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.093 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.6.1_336) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.020 |
High resolution limit [Å] | 1.950 | 4.200 | 1.950 |
Rmerge | 0.064 | 0.040 | 0.501 |
Number of reflections | 31847 | ||
<I/σ(I)> | 12.5 | ||
Completeness [%] | 97.2 | 99.6 | 82.4 |
Redundancy | 3.7 | 3.7 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 9-12% PEG 3350, 0.2 M sodium iodide, 0.1 M Bis-Tris-propane pH 6.5, 0.01 M iron(III) chloride, VAPOR DIFFUSION, HANGING DROP, temperature 293K |